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superscript tm iv first-strand synthesis system kit  (Thermo Fisher)


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    Thermo Fisher superscript tm iv first-strand synthesis system kit
    Superscript Tm Iv First Strand Synthesis System Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/superscript+tm+iv+first+strand+synthesis+kit/bio_rxiv__2025__01__13__632862-143-29-36
    Average 90 stars, based on 1 article reviews
    superscript tm iv first-strand synthesis system kit - by Bioz Stars, 2026-09
    90/100 stars

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    Reverse Transcription:

    Article Title: Acute depletion of human core nucleoporin reveals direct roles in transcription control but dispensability for 3D genome organization
    Article Snippet: .. Reverse transcription was performed by using Superscript TM IV first strand synthesis kit (Thermo Fisher, 18091050), and the random hexamer primer was used. .. Real-time qPCR (RT-qPCR) was performed using SsoAdvanced TM Universal SYBR® Green Supermix (Bio-rad, 172–5274).

    Random Hexamer:

    Article Title: Acute depletion of human core nucleoporin reveals direct roles in transcription control but dispensability for 3D genome organization
    Article Snippet: .. Reverse transcription was performed by using Superscript TM IV first strand synthesis kit (Thermo Fisher, 18091050), and the random hexamer primer was used. .. Real-time qPCR (RT-qPCR) was performed using SsoAdvanced TM Universal SYBR® Green Supermix (Bio-rad, 172–5274).

    Synthesized:

    Article Title: Genome Sequence Variations of Infectious Bronchitis Virus Serotypes From Commercial Chickens in Mexico
    Article Snippet: .. Briefly, cDNAs were synthesized from 10 μL of RNA using random K-8N primer with SuperScript TM IV First Strand synthesis Kit (Invitrogen, USA) and Klenow polymerase (NEB Inc., USA) kits, and then purified using Agencourt AMPure XP beads (Beckman Coulter Life Sciences, USA). .. Purified cDNAs were amplified by Phusion® High-Fidelity PCR Kit (NEB Inc., USA), and used to prepare sequencing libraries by the Nextera TM DNA Flex kit (Illumina, USA), which were then quantified by QubitTM dsDNA HS Assay Kit (Thermo-Fisher Scientific) and Agilent 4150 TapeStation HS D5000 System (Agilent Technologies, Inc.).

    Purification:

    Article Title: Genome Sequence Variations of Infectious Bronchitis Virus Serotypes From Commercial Chickens in Mexico
    Article Snippet: .. Briefly, cDNAs were synthesized from 10 μL of RNA using random K-8N primer with SuperScript TM IV First Strand synthesis Kit (Invitrogen, USA) and Klenow polymerase (NEB Inc., USA) kits, and then purified using Agencourt AMPure XP beads (Beckman Coulter Life Sciences, USA). .. Purified cDNAs were amplified by Phusion® High-Fidelity PCR Kit (NEB Inc., USA), and used to prepare sequencing libraries by the Nextera TM DNA Flex kit (Illumina, USA), which were then quantified by QubitTM dsDNA HS Assay Kit (Thermo-Fisher Scientific) and Agilent 4150 TapeStation HS D5000 System (Agilent Technologies, Inc.).



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